Exploring the dark side of MTT viability assay of cells cultured onto

website mtt stock MTT Cell Proliferation Assay 1 MTT salt : prepare a stock solution of MTT fresh each time just prior to use Add mtt range charts MTT stock solution was added into each well The plates were incubated for 4 h at 37◦C Then, 100 μL of the solubilizing solution was added to each well

mtt stock An MTT stock solution was filter sterilized and kept for no more than 2 weeks at 4°C To start the coloring reaction, stock  Dilute 100X stock MTT solution in media and add 100uL of working MTT solution to each well to achieve mL  Thiazolyl Blue ; 500 mg, USD 30, In-stock ; 1 g, USD 55, In-stock

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